The effects of dead volume on Particle Size(1- 5um) HPLC and the resulting chromatography have been well studied and documented by chemist. The effects of dead volume are critical and significant when using 1-5 um columns as the impact on band broadening greatly increases compared to analytical columns. To optimize the use of narrow bore columns, it is the top priority to reduce the potential dead volume throughout the flow path in the system and the HPLC column.
So, general when dead volume can occur at the HPLC column and instrument, our focus is on the HPLC column end fittings and frits.
