ACN, MeOH, H2O, Isopropanol.
(2) Normal-Phase LC/MS Solvents (for APCI-MS)
Hexane, Methylene Chloride, Acetone, Ethanol.
(3) Compatible LC/MS Buffers and Modifiers:
Formic acid, Acetic Acid, Ammonium Acetate, Ammonium Formate,
Ammonium Hydroxide, Trifluoroacetic Acid (TFA) concentration should
be <0.1%.
Avoid Non-volatile Buffers, Alkali-metal phosphates, borates, etc. that is to say,
ØAcids
l Do not use inorganic acids (may cause source corrosion), Formic and acetic acid are recommended.
Ø Bases:l Do not use alkali metal bases (may cause source corrosion), Ammonium
hydroxide and ammonia solutions are recommended.
hydroxide and ammonia solutions are recommended.
Surfactants (surface active agents): lDetergents and other surface active agents may suppress ionization.
Ø
Trifluoroacetic Acid (TFA): l May enhance chromatographic resolution, but causes ion suppression in both negative and positive ion modes.
Trifluoroacetic Acid (TFA): l May enhance chromatographic resolution, but causes ion suppression in both negative and positive ion modes.
Ø Triethylamine/Trimethylamine (TEA/TMA): May enhance deprotonation for Negative Ion Formation.
