So Happy.... April-21-2008: Today finished the 1st GC assay @ IN.
VIP Tips: (1) The temperature of FID should higher than the final temperature of column, otherwise the baseline would have a little / slight increase.
(2) General Ramp Temp Setting: 50 C (2 min) to 150 C at 10 C/min hold 20 min, then to 250 C at 20 C/min, hold 5 min.
Adjusting the Initial Temperature and Hold Time
To improve the resolution of earlier eluting peaks, decrease the initial temperature or increase the initial hold time. Decreasing the initial temperature usually results in the largest resolution improvement, but analysis times are substantially increased. In addition, cool down times between runs can be significantly increased especially when cooling below 50°C.
However, It is often impossible to cool a GC oven below 35°C in most laboratory environments without using cryogenic oven cooling. The resolution of the later eluting peaks are minimally affected by lowering the initial temperature especially for longer length columns. If excessive resolution is obtained with the original linear temperature program, increase the initial temperature to reduce the resolution and analysis time. The resolution of later eluting peaks may also be
reduced upon increasing the initial temperature.
