5973 Mass Spec failed to pump (1)

On Monday-May-09, found 5973 GC MSD’s failed to pump. Called the Agilent Tech Support, try to isolate the problem. (Bad Hivac Pump? Turbo Pump Over Speed? Leak At Gc Injection-Port? Leak At Mass-Spec Interface? Analyzer Door Thumb Screws To Tight? Or what else?May-10 ~ May12, final, came to a conclusion that the problem may lie in the leak on End-Cap of turbo-molecular pump. However, called the Customer Service of B** Edwards Turbomolecular Pumps , Charged 2-3**, then ordered another “refurbished kit, $5*” from internet.Disconnect the cable between GC and MSD

Take down the cover of EGM

Disconnect 5 wires

Remove the stable screw on the EGM

Pull out the EGM gently

Note: An***, Call me if have any problem, Best

High Pressure Error Message in the 1100 Series Liquid Chromatograph

High Pressure Error Message in the 1100 Series Liquid Chromatograph

1. Ensure that the upper pressure limit in the method is set to a value suitable for the analysis.

2. Disconnect the column and check pressure.

3. If high pressure is observed, even with the column removed, use the injector step commands to switch the injection valve to the bypass mode and monitor the pressure.

4. Open the purge valve. Note pressure. If pressure is high, replace the PTFE frit in the purge valve. If pressure is normal (less than 2 bar), close the purge valve and remove the capillary line from port 1 of the autosampler switching valve.

So, when do I need to change the frit in the Purge Valve of the Solvent Delivery Module (Pump)?

The most common way to determine if the Purge Valve frit needs to be replaced is by opening the Purge Valve and establishing a flow of 1 ml/min of Methanol. If the pressure is more than 7 BAR, the frit should be replaced.

What causes high pressure in my HPLC 1100?

What causes high pressure in my LC?

Probable Causes: 1. Blockage in PTFE frit in the purge valve.
2. Upper pressure limit is set too low.
3. Blockage past the damping unit.
Suggested Actions: 1. Open the purge valve. If the pressure is still high, replace the PTFE frit in the purge valve.
2. Look at the upper pressure limit setting in the software and make sure it is set to a value suitable for the analysis.
3. Check for blockages. (See below)
To Check for blockages, use the split system approach for troubleshooting: Remove the column, pre-column and pre-column filter, if they are installed. 1. If the pressure returns to normal, install a ZDV fitting in place of the column.
a. If the pressure is normal, the column, pre-column or filter was the source of the problem. Replace it.
b. If the pressure is high, look for blockage beyond the column (example: tubing to or from the detector).
2. If the pressure is still high after removing the column, check the tubing from the purge valve to the autosampler. If pressure is normal, use the injector step commands to switch the injection valve of the autosampler to the bypass mode.
a. If the pressure is normal, there is blockage in the autosampler. Check the needle, needle seat, needle seat capillary tubing, and rotor seal.
b. If the pressure is still high, analyze the tubing from the injection valve to the column inlet.