Glucosamine (C6H13NO5) is an amino sugar and a prominent precursor in the biochemical synthesis of glycosylated proteins and lipids.General HPLC Assay@ C18, 4.6x250 mm, 5um.
Mobile Phase: .1 % H3PO4, ACN
Detector Wavelength: 240 nm or 254 nm.
However, could not get a good chromatogram profile.
So low down the H3PO4 to .05%, use NaOH to adjust pH to 3.0, use the 190 wavelength........ Wow..... It works...
So here is a question: How to use the US Pharmacopoeia? 100% follow or made some adjust according your own specific lab facility ?
