Here I would like to emphasis the pressure at HPLC system again.
General lots of chemists would say"Ok, the maximum pressure is 400 Bar or something" We do not need to pay attention to that, because when it excess its maximum pressure, the system could automatically turn off." That is Right, But for me, that is not a good and satisfying answer I want to. Yes, now I want to further to discuss the pressure @ HPLC. Yes, the maximum pressure is 400 Bar. However, that is pressure of flow pathway is not the same as the 400 Bar.When we use H2O/MeOH to wash flow pathway to elute residual analyte or air bubbles, Always CAUTION If pressure is too high, the flow cell may be damaged or broken. When removing air bubbles, set the pump pressure limit to 150 psi (10 atm) or less. Flow cell is easy be damaged or broken when we try to use high flow rate solvents to wash. So Bypass the column, pay attention to the maximum pressure and flow rate, is a Good Lab Practices Standard.
PS: A flow cell rebuilt kit is 400$.
