Monday, August 4, 2008

Basic Operation Procedure of Agilent 6890N-5975 MSD (6)

Quantitative Analysis
Just like the LC-MS ChemStation Software, also need establish a new calibration table:-> calibration /Setup calibration database. (Here are four Quantitative Methods : AREA%,NORM%, ESTD, ISTD.) The different betw GC and GC-MS is that: Need to Qualtilative before Quantitative Analysis.
Load the Method and signals, Select->the Calibrate/Set Up Quantitation
Quantitation Database Globals:Type the Calibration Title, Default Multiplier, Default Sample Concentration, Reference Window/Non-Reference Window, Reference window for Internal STD,Correlation Window(For Scan: 0.05, For SIM:0.025)
Double click the Right button, type the chemical name. If it's a internal STD, Select the Internal icon. Move the “+” to select the targeted ions,click the Left/Right button at the same time. DO NOt forget "Save".
All the internal STD Need to present before compound quantitative
Type the Compound's Cas#. At 2nd Page, there is EnviroQuant for Envi for MDL. If the analyte is water/soil, then don't forget the MDL.
Calibration PlotCalibrate/auto quant setup, -> AutoQuant Setup: Choose Compound Name,Update Calibration,Edit CompoundsQuantitate /Calculate, or data analysis ->Method /Edit Method -> Select Reports-> Quant Report saved with method.Manual Qedit/GraphicsChanging Data States