To MiK***: It depends. -> need to order the column according the analyte in your Lab.
Here is the column info I sued @ 2005' Tech.
2: eclipse c18 150 mm * 4.6 mm ,5 um.
1: zorbax-aq 150 mm * 4.6 mm, 3.5 um.
2: zorbax-aq 250 mm * 4.6 mm, 5 um.
4: zorbax-sq 50 mm * 4.6 mm, 5 um.
3: YMC-am-c18 50 mm * 4.6 mm, 5 um.
4: Waters C18 50 mm *2.0mm ， 5 um.
LC-MS: Prefer use the zorbax-sq 50 mm *4.6 mm,5 um and Waters C18 50 mm *2.0 mm，5 um, HUGE time saving with Syringe pump.
LC only: eclipse c18 150 mm *4.6 mm and zorbax-sq 50 mm *4.6 mm.
