Five Ways to Reduce Acetonitrile Consumption

The current global shortage of Acetonitrile has pushed us to find 5 ways to reduce thedependence on Acetonitrile

Option 1: Reduce column ID:

Reducing the HPLC column ID from 4.6 mm to 3.0 mm can result in > 50 % reduction in mobile phase consumption.

Option 2: Reduce particle size and column dimensions:

* Reduces solvent consumption
* Maintains resolution
* Reduces run time

Option 3: Reduce column length:

Option 4: Substitute Acetonitrile with Methanol:

http://www.phenomenex.com/images/methanal_replacement.gif

Option 5: Solvent recycling (Isocratic separations only)

Reference by Phenomenex.

VIP reference for the Method Transfer....

It's time to clean the Inlet....

Agilent 6890N ECD.
Temp Ramp: 80℃（1min)----20℃/min--- 260℃（15min)----5℃/min---300℃（3min),
Detector Temp: 300℃
Inlet: 250℃
GC Column:HP-5.

Baseline drift?

Maybe the collision energy (CE) is too LOW

(1) Maybe the collision energy is too LOW. The First MS is not full a fragment.
(2) Are you using ESI mode?
(3) If the Molecular weight is 237, for SIM -> Here are three characteristic ions (m/z) 164，149，122 I highly recommend them.
(4) MS-MS (MRM): Need to search online for the product ion and parent ions.
(5) If only need the full scan, why not adjust the concentration of analyte.

Hope it helps.

Agilent MassHunter could meet your request.

Go to MassHunter, File->TIC->.....

m/z (563.10761) is NOT the Molecular Ion? (II)

(1) Try to adjust the the capillary voltage: increase it a little bit each time.
If the the mass response of [M＋H2O －Ｈ]－ is decrease, the mass response of [M-H]- increase: then the m/z (563.10761) definitely is the molecular Ion.
If No such change happened, then the m/z (563.10761) definitely is NOT the Molecular Ion.

(2) From my view, the possibility of ［Ｍ＋Ｈ２Ｏ－Ｈ］( belongs to Molecular ion) is very low. Under ESI (-) negative mode, the trend of forming
the ［Ｍ＋ＨＣＯＯmore high.
Doubt the H2O have that much acidity for forming the ion peak.

m/z (563.10761) is NOT the Molecular Ion? (I)

What is the capillary voltage?
Did you try to increase a little bit?

Where M/Z 425 from? ( II )

Please click the elucidation Scheme for more details:
(Sorry for the confusion in the last post.)

From M/Z (471) to M/Z (425) , maybe belong the loss of Formic Acid (46) .

That is interesting Mass Spectrum, Good luck.

Where M/Z 425 from? ( I )

The molecular weight of your analyte is 548: 1st Mass is 549, 2nd Mass is 489, 3rd Mass are 471, 425.

Here is HUGE challenge for the structural elucidation of your analyte.
From M/Z (471) to M/Z (425) , maybe belong the loss of Acetoxyl M/Z.

But it is NOT right because there are two Acyloxy in your analyte.

Will Update you by Monday Night.