Yohimbine Hydrochloride
C21H26N2O3. HCl
Chromatographic Assay:Mobile Phase:Prepare a mixture of H2O, Dibasic Sodium Phosphate Dihydrate (11.88g/L), and Monobasic Potassium Phosphate Solution (9.08g/L) (355:100:50). Add 4 g of Sodium Dodecyl Sulfate and mix. Add 285 ml of ACN, and mix.
Standard Preparation: USP Yohimbine HCl in MeOH to obtain a solution having a known concentration of about 0.2 mg/ml.
Assay Preparation: Transfer about 50 mg of Yohimbine HCl in 100-mL volumetric flask, dilute with MeOH to Volumn and mix. Transfer 10.0 mL of this stock solution to 25-mL Volumetric flask, dilute with MeOH.
System Suitability Solution: Quantitatively dilute an accurately measured volume of the standard preparation to obtain a solution having a concerntration of .40 ug Yohimbine HCl RS Per ml.
Chromatographic System: C8, 4.6 mm X 150 mm, Detector wavelength 229 nm, tailing factor is NMT 2.5, RSD is NMT 1.0%.
PS: Adapted from U.S. Pharmacopeia / National Formulary， USP30-NF25
