At the on-site setup@Feb-2007, already learned how to tune up the 6410. At March 2007, Mr. B-Cousin** just occasional stopped by Atlanta: then asked him for an update of Mass-Hunter Software. Then I performed an auto-tune on the 6410. After that, the nightmare on the Negative ESI happened. -> Found poor selectivity on the Negative ESI mode ->However the Positive ESI mode worked perfect with a good pass results of tuning. Tried to cal Agilent Tech, but faile to find out a solution.Lots of unknown biofuels in the lab need to identified by PosESI and NegESI, felt huge pressure from the Co-works. Every morning, almost want to smash on the Agilen* 6410 when entered into the lab.
When cool down, checked with the old tune reports with new tune reports: for old tuning solution b4 upgrade the 6410 Masshunter software, tuning peaks@ 112.99, 431.98, 601.98, 1033.99, 1633.95, 2233.91. However when upgrade the software, the tuning m/z is: 112.99, 302.00, 601.98, 1033.99, 1333.97, 1633.95.Then found out problem: maybe did not use the new tuning solution after upgrade the 6410 Masshunter software. Changed the new tuning solution, OMG, perfec. Huge response value for the MET** in the biofules.
Used the old tuning solution after upgrade the 6410 Masshunter software.: expected 302.00m/z , but only found @ 290.26 m/z.
So when the m/z of the analyte is from 230- 350 at NegESI, almost got poor results, made the catalyst guys crazy and cry everyday.See an good GLP on LC/MS tune report @ FDA :http://www.cfsan.fda.gov/~comm/fluoroqu.html#attachb
