For "Dirty Analyst" --- A**P**/ Cin** *-- At least 27 different ingredients in them.
So Question is: What solvents to be used to flush the HPLC lines to remove contaminants?
My Answer is: Prepare solvent mixture consists of IPA and water, 50% each will effectively clean contaminations in the solvent tubes, capillaries, flow cells etc… in an HPLC system. Water will clean salts and other possible contaminants and IPA will clean both polar and non polar possible contaminants. Or just bypass the column with a tubing, then Using a low flow rate of 0.5 ml/min, flush the entire HPLC system for couple of hours to remove any contaminations. Or can also keep the flushing for overnight after preparing enough solvent mixture.
Go Home have a good resting, then when come back at early morning, A new trouble-free HPLC system is ready to go.
