Identification Test: Goldenrod

Goldenrod

Identification Test: Transfer 1 g of sample in 20 ml of Petroleum ether and sonicate 20 min to a suitable flask, filter the sample to remove the Petroleum ether. Allow it dry, add 1 ml diluted HCl and 50 ml Ethyl Acetate, then sonicate 30 min. Filter the sample to remove the solvent, allow the residue to dry, add 2 ml MeOH, the residue dissolve.

Apply separate 10-ul portions of this solution and of a standard solution of USP Chlorogenic Acid RS in MeOH containing 0.5 mg per ml to the starting line of a thin-layer chromatographic plate (See chromatography <621>) coating with 0.25-mm layer of chromatographic silica gel mixture. Allow the spots dry, and develop the chromatogram in an unsaturated chamber with a solvent system consisting of a mixture of Toluene-Ethyl Acetate-Formic Acid-Glacial Acetic Acid-H2O is 1/ 15/ 1/ 1/ 2) until the solvent front have moved about 3/4 of the length of the plate. Remove the plate from chamber, air-dry and view under 365 nm long-wavelength UV-Light: the Rf value, color of the principal spot obtained from the test solution corresponds to that obtained from the standard solution.

From China Pharmacopoeia 2005 Volume 1- P218 .

If need,
HPLC Analysis of the flavonoids in pharmaceutical preparations from Solidago canadensis
See the paper: J. Pharm. Biomed. Anal. 32 (2003) 1045-2-53.
Results: NLT 0.20 % Chlorogenic acid in goldenrod....

Done....Night--- 11:05 PM July-15-2008