The linear-solvent-strength (LSS) model for gradient elution is based on an approximation for isocratic retention in RP-LC as a function of solvent strength.
Bonus Question: When run out of mobile phase, what is the consequence for the HPLC system...A: all the hydraulic pathway would dry out
B: the air come into the pump, hydraulic pathway
C: the air would come into Column
D: the air could come into flow cell.
or what else....
( Tip: High school physical knowledge: air-pump...or try to recall when onsite setup the HPLC system, what need to do b4 start the pump at the first time?
Cool, one coke for the right answer, Mountain Dew
