Assay of Caffeic Acid in Dandelion 
Dandelion (Herb Taraxaci)
Identification: TLC.
Column : C18, 250 x 4.6, 5 um
Mobile Phase A: 0.01 M NaH2PO4, pH 3.8-4.0.
B : MeOH. A/B is 70/30.
Flow Rate: 1.0 ml/min
Detector Wavelength: 323 nm, 30 degree C.
Standard Prep: 7.5 mg std in 50 ml MeOH, shake & dissolve well, pipet 2 ml to 1o ml volumetric flask, bring to volume with MeOH.
Sample Prep: Weigh 1 g in 50 ml, fill to volume with 5% HOAc in MeOH.
Results: HPLC assay of Caffeic acid NLT 0.020 %. Correct me if i am wrong, to my best knowledge, there is some connection between Caffeic acid and carcinogenicity.
