Electrospray Summary

1. Analyte type:

1.1 Preformed ions (acids and bases)
1.2 Polar neutrals
1.3 Multiply charged ions of biopolymers
1.4 NMT<100>

2. Typical flow rates: low down - 1.0 ml/min
3. Promote ionization:
3.1 Correct pH
3.2 Favorable HPLC solvent composition
3.3 Post-column addition of reagents

4. Soft ionization technique

5. Typical applications: Drugs, Sugars, Peptides, Proteins, Oligonucleotides

Here is another question: Why GC-MS have a NIST/EPA/NIH Mass Spectral Library 2005 or 2008.? that is "Standard" Mass Spectral for GC-MS, it help newbie chemist would master the elucidation skill of Mass Spectral in 3 weeks.

However, Why LC-MS do not have any LC-MS Mass Spectral Library?
Answer: give me one Coke, i will tell you..... or call the Agilent/Waters/Varian/AB/PE Technical Support, they would answer...

Tips: Look at above # 4: comparing the ionization technique.
for LC-MS: ESI, APCI, APPI ( they are Soft technique), the ionization energy@interface different, the mass fragment different, ---- so not a universal mass library. Or or call the Agilent/Waters/Varian/AB Technical Support, ask them why develop so many "New and Individual" Patent ( on their own instrumentation only) on LC-MS ionization technique...

For GC-MS: EI ( Hard ionization technique), the Ionization energy (from 5 to 241.5 eV) is stable, so the molecular fragment should be the same....that is the reason why NIST/EPA/NIH have Mass Library.

It is so simple: understand the basic ionization technique, should answer.