(1) Weigh 1 g of sample in 5ml of Petroleum ether ( 30-60 degree C) into 10 ml tube, shake the tube 2 or 3 times in 10 hrs. Let it still, pipet the upper clear solution 1 ml in to a evaporating dish. Allow the solvent to evaporate, add 1 ml MeOH, the residues dissolve.
(2) then add 2% 3,5-Dinitrobenzoic Acid (in MeOH) testing solution 2-3 drops, and 2 drops of Sodium Hydroxide Solution (saturated dissolves in Methanol), display Purple & red.
From China Pharmacopoeia 2005 - P28.
Mass Spectra of Ferulic Acid (CAS# 537-98-4)
C10H10O4
Source Temperature: 150 °C,
Sample Temperature: 140 °C,
DIRECT, 75 eV,
Mass of molecular ion: 194.
Peak Data:
51.0 5.5
77.0 7.0
105.0 5.7
133.0 13.6
145.0 5.0
161.0 7.4
176.0 5.2
177.0 8.6
179.0 19.0
193.0 6.1
194.0 100.0
195.0 11.1
Low down the flow rate and injection concentration.
